THE REFRACTOMETRIC DETERMINATION OF SERUM PROTEINS
نویسندگان
چکیده
منابع مشابه
The Refractometric Determination of Hemoglobin
The standard method of determining hemoglobin concentration in blood is based on a measurement of oxygen capacity and Htifner’s factor, 1.34 cc. of oxygen per gm. of hemoglobin. Unfortunately, the Van Slyke and the Haldane machines do not give the same results for the oxygen capacity. The difference is over 10 per cent of the total, and there is no agreement as to which method is correct. The c...
متن کاملNew refractometric methods for the determination of total proteins in serum and in urine.
New, precise, physical methods for the determination of total protein in serum or plasma and in urine are described. They are based upon the refractometric determination of total solids in these fluids before and after protein has been removed by coagulation and, in ordinary use, they require no special standardization or calibration. The urine method is applicable primarily to protein concentr...
متن کاملThe Refractometric Determination of Hemoglobin
The standard method of determining hemoglobin concentration in blood is based on a measurement of oxygen capacity and Htifner’s factor, 1.34 cc. of oxygen per gm. of hemoglobin. Unfortunately, the Van Slyke and the Haldane machines do not give the same results for the oxygen capacity. The difference is over 10 per cent of the total, and there is no agreement as to which method is correct. The c...
متن کاملThe Refractometric Determination of Hemoglobin
The standard method of determining hemoglobin concentration in blood is based on a measurement of oxygen capacity and Htifner’s factor, 1.34 cc. of oxygen per gm. of hemoglobin. Unfortunately, the Van Slyke and the Haldane machines do not give the same results for the oxygen capacity. The difference is over 10 per cent of the total, and there is no agreement as to which method is correct. The c...
متن کاملLxxviii. the Determination of Diffusion Constants of Proteins by a Refractometric
IN the calculation of the molecular weights of proteins from sedimentation data it is essential that their diffusion constants should be known accurately. At first diffusion constants were measured from the spreading at the sedimenting boundary in the ultracentrifuge [Svedberg, 1925, 1, 2] and the concentration changes were determined from measurements of the light absorption of the solutions. ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1923
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)85653-8